Methods of cancer treatment using tumor antigen-specific T cells
AssigneesSYZ Cell Therapy Co., HRYZ (Shenzhen) Biotech Co.
InventorsXiangjun Zhou, Yanyan Han, Xihe Chen, Xiaoling Liang
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Abstract
The present application provides methods of preparing tumor antigen-specific T cells comprising enriching activated T cells from a first co-culture comprising a first population of antigen-loaded dendritic cells loaded and a population of T cells, and co-culturing the enriched activated T cells with a second population of antigen-loaded dendritic cells. Also provided are methods of treating cancer in an individual using the tumor antigen-specific T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.
Claims20
A method of treating a cancer in an individual, comprising:
- a) a first co-culturing step, comprising co-culturing a first population of dendritic cells loaded with a plurality of tumor antigen peptides with a population of T cells to obtain a first co-culture comprising activated T cells;
- b) an enrichment step, comprising contacting the first co-culture with antigen presenting cells (APCs) loaded with the plurality of tumor antigen peptides to obtain a stimulated co-culture, and isolating from the stimulated co-culture an enriched population of activated T cells using a ligand that specifically recognizes a cytokine expressed by the activated T cells;
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- c) a second co-culturing step, comprising co-culturing the enriched population of activated T cells with a second population of dendritic cells loaded with one or more tumor antigen peptides from the plurality of tumor antigen peptides to obtain a population of tumor antigen-specific T cells;
- and
- d) administering to the individual an effective amount of the tumor antigen-specific T cells, wherein the individual has previously received an immunotherapy.
Dependencies are inferred from the claim text.