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AssigneesShandong Institute for Food and Drug Control, Shandong University, Avanc Pharmaceutical Co.,Ltd
InventorsFeng Shi, Ruiqing Xian, Liping Gong, Weijian Wang, Lianli Chi, Congcong Wang, Baojian Hang, Xunjie Zhang, Li Nie, Fengshan Wang
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The present invention relates to the field of chemical analysis detection and application, in particular to a marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas and an application thereof. The amino acid sequence of the marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas is TLCAGVMEGGIDTCNR. Characterizing the source of species and a content of the SVTLEs in a to-be-detected sample by using the marker peptide includes the following steps of: pretreating the to-be-detected sample by trypsin through enzymolysis, and taking a supernatant of an enzymolysis liquid as a test solution; and injecting the test solution and a reference solution into a liquid chromatography-mass spectrometer, and selecting a qualitative ion pair and a quantitative ion pair for detecting the source of species and a content of the SVTLEs in the to-be-detected sample.
A marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas, wherein an amino acid sequence of the marker peptide is TLCAGVMEGGIDTCNR.
Dependencies are inferred from the claim text.