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US 11,467,073 B2Granted
Method for concentrating extracellular vesicles
AssigneeSysmex Corporation
InventorsKoya Yamawaki, Yuki Aihara
Life of this patent
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Abstract
Disclosed is a method for concentrating extracellular vesicles, comprising preparing a mixture comprising a first fraction and a second fraction by mixing a liquid sample comprising extracellular vesicles, a polysaccharide, and a polyether having an average molecular weight of 20,000 or less, wherein the first fraction comprises a higher concentration of extracellular vesicles than the second fraction, and the first fraction comprises a higher concentration of extracellular vesicles than the liquid sample.
Claims10
A method for concentrating extracellular vesicles, comprising:
- preparing a mixture comprising a first fraction and a second fraction, wherein said mixture is prepared by mixing a liquid sample comprising extracellular vesicles with a dextran having a weight average molecular weight of between 100,000 and 700,000, and with a polyethylene glycol having a number average molecular weight of between 8,980 and 18,400,
- wherein the first fraction comprises a higher concentration of extracellular vesicles than the second fraction, and the first fraction comprises a higher concentration of extracellular vesicles than the liquid sample,
- and wherein the liquid sample is blood serum, blood plasma, lymph fluid, urine, ascites, pleural effusion, cerebrospinal fluid, or interstitial fluid.
Dependencies are inferred from the claim text.