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US 11,466,309 B2Granted
Mechanically-strained oligonucleotide constructs and methods of using the same
AssigneeBoard of Trustees of the University of Arkansas
InventorYong Wang
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Abstract
Disclosed herein are mechanically-strained oligonucleotide constructs comprising two oligonucleotides that when hybridized results in a bent double-stranded oligonucleotide. The constructs may be used to probe oligonucleotide interactions with an analyte to detect interactions with metal ions or compounds.
Claims11
A method for detecting the interaction of an analyte and a mechanically-strained oligonucleotide construct, the method comprising:
- providing a solution, the solution comprising the mechanically-strained oligonucleotide construct and the analyte;
- detecting at least one detectable characteristic of the mechanically-strained oligonucleotide;
- wherein the mechanically-strained oligonucleotide construct comprises a first oligonucleotide, the first oligonucleotide comprising a first domain and a second domain in order from 5′ to 3′, and a second oligonucleotide, the second oligonucleotide comprising a first complementary domain, a single-stranded domain, and a second complementary domain in order from 5′ to 3′;
- wherein the first domain and the first complementary domain are completely or partially complementary and capable of completely or partially hybridizing with each other;
- wherein the second domain and the second complementary domain are completely or partially complementary and capable of completely or partially hybridizing with each other;
- and
- wherein the at least one detectable characteristic of the mechanically-strained oligonucleotide is (i) a concentration of the mechanically-strained oligonucleotide construct, a component of the mechanically-strained oligonucleotide construct, a higher-order construct, or any combination thereof, and/or (ii) a spectroscopic feature.
4 more elements
Dependencies are inferred from the claim text.