Method for preparing phosphorylated keto polyols, derivatives thereof and uses thereof

A method for preparing a phosphorylated keto polyol of formula (I)

• or a cyclic hemiketal isomer thereof, comprising a step of reacting dihydroxyacetone phosphate (DHAP) with a ketone of formula (III) selected from a 1,2-diketone and a ketone having a substituent X at a position alpha of the carbonyl group:
• in the presence of a class II DHAP aldolase,
• wherein R1 and R2 are such that:
• the molecular weight of the ketone of formula (III) is less than 600 g.mo−1,
• neither R1 nor R2 are H, and
• the carbonyl group shown in formula (III) is included in a moiety selected from:
• and
• wherein X is selected from the group consisting of
• OH, F, Cl, Br, I, —N3, cyano, nitro, —COOH, —SO3H, —C(F)3, —C(Cl)3, —C(Br)3, —C(I)3,
• —NHC(═O)R, —NHC(═O)OR, —OR, —SR, —SO2R, —C(═O)R, —C(═O)OR, —C(═O)NHR, —OC(═O)OR, —C(═O)SR, wherein R is a substituted or unsubstituted C1-C10 alkyl, a substituted or unsubstituted C5-C10 aryl or a substituted or unsubstituted C4-C10 heteroaryl group,
• —N(R3)3+ wherein each R3 is independently selected from H, C1-C10 alkyl and C5-C10 aryl,
• OP(═O)(R4)2 and P(═O)(R4)2 wherein each R4 is independently selected from H, a substituted or unsubstituted C1-C10 alkyl, a substituted or unsubstituted C5-C10 aryl, a substituted or unsubstituted C4-C10 heteroaryl group, substituted or unsubstituted C1-C10 alkoxy and substituted or unsubstituted C5-C10 aryloxy.