US 11,466,288 B2 · Methods for reproducing plants asexually and compositions thereof

Methods for reproducing plants asexually and compositions thereof

AssigneesPioneer Hi-Bred International, Inc., Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional (CINVESTAV)

InventorsShai J. Lawit, Marc C. Albertsen, Tim Fox, William Gordon-Kamm, Michelle M. Van Allen, Jean-Philippe Vielle Calzada, Carlos Barragan Rosillo, Edgar Demesa Arevalo, Carlos Gonzalez Chavez, Elvira Hernandez Lagana, Gloria Leon Martinez, Nidia Sanchez Leon, Daniel Rodriguez Leal, Isaac Rodriguez Arevalo, Jaime Padilla Calzada

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Abstract

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Claims2

Dependent claims

A method for enriching asexual embryos and endosperm in a seed population of a dicot plant, the method comprising:

providing a dicot plant comprising an asexual female gametophyte defective for RNA-dependent DNA methylation (RdDM) activity, wherein the RdDM activity is defective for RDR6 (RNA-DEPENDENT RNA POLYMERASE 6), and wherein the asexual female gametophyte of the dicot plant is also disrupted for activity in Fertilization Independent Embryo (FIE), wherein the dicot plant is Arabidopsis, canola, cauliflower, cabbage, broccoli, turnip or radish, wherein the seeds formed comprise parthenogenically-derived clonal embryos.

Dependencies are inferred from the claim text.

Facts

Application

15/512,629Filed 21 Sep 2015 · us provisional application

Priority

22 Sep 2014

Published

US 2017/0290279 A112 Oct 2017

Granted

11 Oct 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

A01H 4/005
Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor: methods for micropropagation; Vegetative plant propagation…
C12N 15/8287
With agronomic traits, e.g. crop yield: for fertility modification, e.g. apomixis
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 6/202
Brassicaceae, e.g. canola, broccoli or rucola: brassica napus [canola]