Compositions and methods to increase resistance to phytophthora in soybean
AssigneesCorteva Agriscience LLC, Purdue Research Foundation
InventorsJianxin Ma, Rajat Aggarwal, Liyang Chen, Weidong Wang, Oswald Crasta, Jonathan Myrvold
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Abstract
Provided are compositions comprising polynucleotides encoding Rps genes, and soybean plants or soybean seeds comprising the compositions and exhibiting resistance to Phytophthora. Additionally, various methods for employing the polynucleotides to increase resistance to Phytophthora are also provided herein.
Claims8
A method of introgressing a Phytophthora Root and Stem Rot (PRSR) resistance gene into a soybean plant, said PRSR resistance gene comprising a polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:
- 2, said method comprising;
- i) crossing a PRSR resistant soybean plant comprising a heterologous polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:
- 2 with a second soybean plant to produce progeny;
- ii) screening said progeny with a nucleic acid marker to detect progeny comprising a polymorphism genetically linked to said PRR resistance gene;
- and
- iii) selecting progeny that comprise said polymorphism to obtain soybean plants that contain said PRSR resistance gene.
A method for generating a soybean plant having enhanced Phytophthora resistance, the method comprising:
- (a) introducing a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 2 into the cells of a soybean plant, and
- (b) identifying a generated soybean plant that comprises the cells expressing said introduced polynucleotide, wherein the identified plant has increased resistance to a Phytophthora infection as compared to a soybean plant not comprising the introduced polynucleotide.
Dependencies are inferred from the claim text.