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AssigneeUniversity of Florida Research Foundation, Inc.
InventorsJulie A. Maupin-Furlow, Nathaniel L. Hepowit, Lana McMillan
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The invention pertains to a PPA from a microorganism belonging to the family Halobacteriaceae (HPPA), for example, a PPA from Haloferax volcanii. The HPPA provided by the invention is soluble, thermostable and active at high concentrations of salt and/or organic solvent. An embodiment of the invention provides a method of increasing the rate of a reaction by adding an HPPA to the reaction mixture, wherein the reaction produces PPi, for example, an enzymatic reaction, and wherein the reaction is carried out at moderately high temperature and/or low water activity. Further embodiments of the invention provide an assay to detect the PPi released during a reaction which produces PPi by adding an HPPA to convert the PPi in to Pi and measuring the resultant Pi. The invention further pertains to an assay to monitor a reaction which produces PPi in the presence or the absence of an HPPA.
A method comprising:
A composition comprising a reaction mixture that removes inorganic pyrophosphates (PPi) from a prior reaction and produces inorganic phosphates (Pi), the reaction mixture comprising (1) an inorganic pyrophosphatase from a microorganism belonging to family Halobacteriaceae (HPPA) of SEQ ID NO:
Dependencies are inferred from the claim text.