Modified PEDV spike protein
OwnerVeljko Nikolin, Andreas Galleiinventors
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Abstract
The present invention relates to a nucleic acid molecule encoding a modified porcine epidemic diarrhea virus (PEDV) spike (S) protein and the PEDV (S) protein thereof. Further, the present invention relates to immunogenic compositions comprising said modified PEDV spike protein and methods for immunizing a subject comprising the administration of said immunogenic composition to a subject.
Claims15
A nucleic acid molecule encoding a porcine epidemic diarrhea virus (PEDV) spike (S) protein selected from the group consisting of the following (a) and (b):
- (a) a genotype 2a (G2a) PEDV S protein having at least one mutation, wherein
- the leucine residue at amino acid position 900 is substituted by an amino acid residue other than a leucine residue, and/or
- the leucine residue at amino acid position 901 is substituted by an amino acid residue other than a leucine residue,
- the tyrosine residue at amino acid position 1377 is substituted by an amino acid residue other than a tyrosine residue, and/or
- the histidine residue at amino acid position 1384 is substituted by an amino acid residue other than a histidine residue,
- wherein the numbering of the amino acid positions refers to the amino acid sequence of wild type G2a PEDV S protein of SEQ ID NO:2 or SEQ ID NO:39,
- (b) a genotype 2b (G2b) PEDV S protein having at least one mutation, wherein
- the leucine residue at amino acid position 897 is substituted by an amino acid residue other than a leucine residue, and/or
- the leucine residue at amino acid position 898 is substituted by an amino acid residue other than a leucine residue,
- the tyrosine residue at amino acid position 1374 is substituted by an amino acid residue other than a tyrosine residue, and/or
- the histidine residue at amino acid position 1381 is substituted by an amino acid residue other than a histidine residue,
- wherein the numbering of the amino acid positions refers to the amino acid sequence of wild type G2b PEDV S protein of SEQ ID NO:3.
A nucleic acid molecule encoding a porcine epidemic diarrhea virus (PEDV) spike (S) protein comprising the amino acid sequence
- (SEQ ID NO:
- 1)
- RSX1IEDX2X3,
- wherein R is the conserved arginine residue of the S1/S2 cleavage site of said PEDV S protein, X1 can be any amino acid residue, and wherein
- X2 is an amino acid residue other than a leucine residue and X3 is an amino acid residue other than a leucine residue;
- wherein said PEDV S protein further comprises the amino acid sequence
- (SEQ ID NO:
- 6)
- X1X2X3FX4KX5X6X7X8,
- wherein X8 is the C-terminal amino acid residue of said PEDV S protein or the amino acid residue at the −2 position relative to the C-terminal amino acid position of said PEDV S protein,
- X2 to X5, X7 and X8 can be any amino acid residue, and wherein
- X1 is an amino acid residue other than a tyrosine residue and X6 is a histidine residue.
Dependencies are inferred from the claim text.