US 11,459,601 B2 · Diagnostic device and system

Abstract

A portable diagnostic device has a lysate stage with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages are linked with the lysate vessel, each with a reservoir linked by channels. The final stage has a sensor for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.

Claims25

Dependent claims

A portable diagnostic device comprising:

a housing,
assay stages, each with a reservoir linked by channels,
at least one of the assay stages including a sensor for detecting target analyte,
an inlet for receiving a sample and including:
a sample lysing agent,
an outlet port to the assay stages, the outlet port having a wax or oil plug having embedded magnetic beads for attachment by a probe to target analyte.

3 more elements

A portable diagnostic system comprising a device and a reader, wherein the device comprises:

a housing, assay stages, each with a reservoir linked by channels, at least one of the assay stages including a sensor for detecting target analyte, an inlet for receiving a sample and including:
a sample lysing agent an outlet port to the assay stages, the outlet port having a wax or oil plug having embedded magnetic beads for attachment by a probe to target analyte, and wherein the reader comprises:
a support for receiving the device;
magnets on a drive to convey the magnetic beads through the assay stages of the device;
a heat source;
a means for communicating with the sensor of the device, and
a controller.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/647,795Filed 6 Sep 2018

Priority

EP 1719215320 Sep 2017

Published

US 2020/0216884 A19 Jul 2020

Granted

4 Oct 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12Q 1/6816
Hybridisation assays: characterised by the detection means
C12Q 1/6825
Characterised by the detection means: nucleic acid detection involving sensors
B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01L 3/502784
Characterised by multiphase flow arrangements: specially adapted for droplet or plug flow, e.g. digital microfluidics

Diagnostic device and system

Life of this patent

Diagnostic device and system

Life of this patent

Abstract

Claims25