Processes for the production of cannabinoids from a carbon source precursor
AssigneeSyntiva Therapeutics, Inc.
InventorsAdam Davidovich, Brian Chan, Neil Gupta, Alim Ladha
Life of this patent
Estimating the term…
Abstract
A method is provided for biosynthetic production of cannabinoids in microorganisms from a carbon source precursor. This method describes the genetic modifications needed to engineer microorganisms to produce cannabinoids as well as a method for identifying and quantifying cannabinoids from fermentation broth. A system is also provided for tuning the method to produce different cannabinoids of interest by systematically modulating the enzymes encoded by the genetic modifications introduced in the microorganism.
Claims10
A method for producing at least one cannabinoid from a carbon source precursor, comprising:
- genetically modifying a microorganism to express enzymes olivetol synthase, olivetolic acid cyclase, and CsPT1 for converting the carbon source precursor into at least one cannabinoid within the genetically modified microorganism wherein the microorganism is E. coli comprising a recombinantly incorporated mutated FadD gene having a nucleotide sequence of SEQ ID NO:
- 10 at the genomic location of a FadE gene of the microorganism to express the mutated FadD enzyme and simultaneously knock out the FadE gene of the microorganism, wherein the olivetol synthase comprises the amino acid sequence of SEQ ID NO:
- 2, wherein the olivetolic acid cyclase comprises the amino acid sequence of SEQ ID NO:
- 4, and wherein the CsPT1 comprises the amino acid sequence of SEQ ID NO:
- 6.
A method for producing at least one cannabinoid from a carbon source precursor, comprising:
- genetically modifying a microorganism to express enzymes olivetol synthase, olivetolic acid cyclase, and CsPT1 and converting the carbon source precursor into at least one cannabinoid within the genetically modified microorganism, wherein the microorganism is S. cerevisiae and has been transformed with a plasmid to express a polypeptide encoding olivetol synthase having the amino acid sequence of SEQ ID NO:
- 2, a polypeptide encoding olivetolic acid cyclase having the amino acid sequence of SEQ ID NO:
- 4, a polypeptide encoding CsPT1 having the amino acid sequence of SEQ ID NO:
- 6, and further comprising a Glutathione-S-Transferase (GST) solubility tag with a thrombin cleavage site having the amino acid sequence of SEQ ID NO:
- 8 in the same open reading frame as CsPT1.
Dependencies are inferred from the claim text.