Method for the microbial production of specific natural capsaicinoids

A method of producing a capsaicinoid glucoside of interest, said method comprising:

• providing a transformed microbe culture capable of expressing 1) an ACS enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO:
• 1 and/or a DNA sequence similarity of at least 75% with SEQ ID NO:
• 3, 2) a CS enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO:
• 2 and/or a DNA sequence similarity of at least 90% with SEQ ID NO:
• 4, and 3) a CaUGT2 enzyme having either a protein sequence similarity of at least 90% with SEQ ID NO:
• 6 and/or a DNA sequence similarity of at least 75% with SEQ ID NO:
• 5;
• incubating the transformed microbe culture to induce expression of the ACS enzyme, the CS enzyme, and the CaUGT2 enzyme;
• feeding a specific fatty acid precursor to said transformed microbe culture.