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US 11,454,629 B2Granted
In situ-generated microfluidic assay structures, related kits, and methods of use thereof
AssigneeBerkeley Lights, Inc.
InventorsKristin G. Beaumont, Peter J. Beemiller, Volker L. S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
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Abstract
In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
Claims21
A microfluidic device comprising:
- an enclosure comprising a substrate and microfluidic circuit material, the enclosure defining a flow region and a sequestration pen, each located within the enclosure;
- and
- an in situ-generated capture structure disposed within the sequestration pen, wherein the in situ-generated capture structure comprises a solidified polymer network, wherein the solidified polymer network comprises a functionalized site;
- and further wherein the in situ-generated capture structure permits export of a micro-object from the microfluidic device.
1 more element
Dependencies are inferred from the claim text.