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US 11,454,588 B2Granted
Fluorescent spectrum correcting method and fluorescent spectrum measuring device
AssigneeSony Corporation
InventorsMasaya Kakuta, Koji Futamura, Yoshitsugu Sakai, Yasunobu Kato
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Abstract
A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.
Claims19
A flow cytometry method, comprising:
- detecting, by a plurality of detectors, a first fluorescent spectrum from each of a plurality of cells labelled with a plurality of fluorescent pigments under a first condition;
- separating, by an analysis circuit, the detected first fluorescent spectrum into a second fluorescent spectrum for each fluorescent pigment of the plurality of fluorescent pigments by using a reference spectrum, wherein
- the reference spectrum is calculated from a stored fluorescent spectrum data detected under a second condition, and
- the first condition is different from the second condition;
- and
- generating, by the analysis circuit, a density plot based on the second fluorescent spectrum for each fluorescent pigment to classify the plurality of cells.
3 more elements
A flow cytometry system, comprising:
- a plurality of detectors configured to detect a first fluorescent spectrum from each of a plurality of cells labelled with a plurality of fluorescent pigments under a first condition;
- a memory configured to store fluorescent spectrum data detected under a second condition;
- and
- an analysis circuit configured to:
- separate the detected first fluorescent spectrum into a second fluorescent spectrum for each fluorescent pigment of the plurality of fluorescent pigments by using a reference spectrum,
- wherein
- the first condition is different from the second condition, and
- the reference spectrum is calculated from the fluorescent spectrum data detected under the second condition;
- and
- generate a density plot based on the second fluorescent spectrum for each fluorescent pigment to classify the plurality of cells.
7 more elements
A flow cytometry, comprising:
- a plurality of detectors configured to detect a first fluorescent spectrum from a plurality of cells labelled with a plurality of fluorescent pigments under a first condition, wherein the plurality of cells flows in a flow path;
- and
- an analysis circuit configured to:
- separate the detected first fluorescent spectrum into a second fluorescent spectrum for each fluorescent pigment of the plurality of fluorescent pigments by using a reference spectrum, wherein
- the reference spectrum is calculated from a stored fluorescent spectrum data detected under a second condition, and
- the first condition is different from the second condition;
- and
- generate a density plot based on the second fluorescent spectrum for each fluorescent pigment to classify the plurality of cells.
5 more elements
Dependencies are inferred from the claim text.