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US 11,454,577 B2Granted
Method and apparatus for collecting biomolecules from specific region of tissue section
AssigneeHitachi, Ltd.
InventorMasataka Shirai
Life of this patent
Estimating the term…
Abstract
Provided is, in a sample of a cell or a micro region at a position designated in a microscope image of a tissue section, an apparatus and a method for analyzing a biomolecule, which are capable of collecting and analyzing a biomolecule in a single cell or in a micro region without damaging surrounding cells.
Claims15
An apparatus for collecting a biomolecule from a specific region of a tissue section, comprising:
- a stage on which a tissue section is fixed;
- a microscope system configured to acquire an optical image of the tissue section;
- an energy application system configured to disrupt at least a part of a specific region of the tissue section;
- a dispensing pipette system configured to form a droplet on the tissue section;
- and
- a computer system configured to designate the specific region on the tissue section on the optical image acquired by the microscope system,
- wherein a biomolecule from the specific region being disrupted is dissolved or dispersed in the droplet.
4 more elements
A method for collecting a biomolecule from a specific region of a tissue section, comprising:
- microscopically observing a tissue section;
- designating a specific region of the tissue section;
- dispensing a recovery solution on the tissue section so that a droplet to be formed covers the specific region, and forming a droplet;
- applying energy to at least a part of the specific region in a state where the droplet is present on the tissue section;
- and
- recovering the droplet.
3 more elements
A method for collecting a biomolecule in a specific cell of a cultured cell adhered onto a substrate, comprising:
- forming a water-repellent thin film on a cultured cell adhered onto a substrate;
- microscopically observing the cultured cell;
- designating a specific cell of the cultured cell;
- dispensing a recovery solution on the water-repellent thin film so that a droplet to be formed covers the specific cell, and forming a droplet;
- applying energy to at least a part of the specific cell in a state where the droplet is present on the water-repellent thin film;
- and
- recovering the droplet.
4 more elements
Dependencies are inferred from the claim text.