Method of detecting bacterial infection in a biological sample
AssigneeTrubac, LTD.
InventorsErnest A. Azzopardi, Rosa Sofia Rodrigues Teixeira
Life of this patent
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Abstract
A method of indicating the presence of a bacterial infection in a biological sample is provided. The method detects a marker for infection by providing a device, the device including a biosensor, an interaction arising between the biosensor and the marker when the marker is present in the biological sample. Contacting at least a part of the biological sample with the biosensor of the device, therefore, provides analysis of the biological sample with respect to the marker by detecting for the interaction between the biosensor and the marker. A preferred marker is the enzyme amylase.
Claims19
A method of indicating the presence of a bacterial infection in a biological sample obtained from a wound of a subject, other than a pancreatic wound or oral digestive wound, the method comprising:
- a. providing a device comprising a biosensor, wherein the biosensor includes an α-amylase specific antibody and an interaction arises between the α-amylase specific antibody and α-amylase when the α-amylase is present in the biological sample;
- b. contacting at least a part of the biological sample with the biosensor of the device;
- c. analysing the at least a part of the biological sample with respect to the α-amylase by detecting for the interaction between the α-amylase specific antibody and the α-amylase;
- d. indicating an amount of the α-amylase present in the biological sample using the device;
- e. repeating steps b.-d. on a blood, plasma, or serum sample obtained from the subject, thereby indicating an amount of α-amylase present in the blood, plasma, or serum sample;
- and
- f. comparing the amount of α-amylase in the biological sample with the amount of α-amylase in the blood, plasma, or serum sample, and where the difference in the amount of α-amylase in the biological sample compared with that in the blood, plasma, or serum sample is elevated above 140 IU/L, concluding the elevated amount of α-amylase is indicative of a bacterial infection present in the biological sample.
A method of indicating the presence of a bacterial infection in a biological sample obtained from a wound of a subject, wherein the wound is not associated with a known pancreatic, salivary, or macroamylasemia pattern of amylase distribution, the method comprising:
- a. providing a device comprising a biosensor, wherein the biosensor includes an α-amylase specific antibody and an interaction arises between the α-amylase specific antibody and α-amylase when the α-amylase is present in the biological sample;
- b. contacting at least a part of the biological sample with the biosensor of the device;
- c. analysing the at least a part of the biological sample with respect to the α-amylase by detecting for the interaction between the α-amylase specific antibody and the α-amylase;
- d. indicating an amount of the α-amylase present in the biological sample using the device;
- e. repeating steps b.-d. on a blood, plasma, or serum sample obtained from the subject, thereby indicating an amount of α-amylase present in the blood, plasma, or serum sample;
- and
- f. comparing the amount of α-amylase in the biological sample with the amount of α-amylase in the blood, plasma, or serum sample, and where the difference in the amount of α-amylase in the biological sample compared with that in the blood, plasma, or serum sample is elevated above 140 IU/L, concluding the elevated amount of α-amylase is indicative of a bacterial infection present in the biological sample.
Dependencies are inferred from the claim text.