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US 11,453,903 B2Granted
Production of activated clostridial neurotoxins
AssigneeIpsen Biopharm Ltd.
InventorsLaura Lovelock, Daniel Kwan, Peter Daniel Horrocks, Malgorzata Field, Philip Marks
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Abstract
Method of producing an activated clostridial neurotoxin. Composition comprising an activated clostridial neurotoxin. Method of treatment using a composition comprising an activated clostridial neurotoxin.
Claims12
A method for producing a composition comprising an activated clostridial neurotoxin and less than 10% A single-chain or truncated clostridial neurotoxin, the method comprising:
- contacting a single-chain clostridial neurotoxin comprising a BoNT/E activation loop with trypsin at a concentration of 0.5 to 3 μg per mg of clostridial neurotoxin and a pH between 6 and 7 for a duration of 15 to 25 hours to convert the single-chain clostridial neurotoxin into di-chain clostridial neurotoxin;
- and
- contacting the di-chain clostridial neurotoxin with a mixed mode chromatography resin.
Dependencies are inferred from the claim text.