Method of producing protein
AssigneeToray Industries, Inc.
InventorsKoji Kobayashi, Shingo Hiramatsu, Katsushige Yamada
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Abstract
A method produces a protein using a filamentous fungus, in which decrease in dissolved oxygen saturation during culture of the filamentous fungus can be suppressed even when the culture is scaled up. The method of producing a protein includes culturing a fungus belonging to the genus Trichoderma whose BXL1 gene was disrupted, using a biomass containing cellulose and xylan as an inducer. The use of the BXL1 gene-disrupted fungus belonging to the genus Trichoderma enables suppression of the decrease in dissolved oxygen saturation even when xylose and cellulose are used as inducers.
Claims4
A cellulase composition comprising hydrolases that hydrolyze glycosidic linkages and are obtained from a culture medium of BXL1 gene-disrupted Trichoderma having the following characteristics (a) to (d):
- (a) the cellulase composition has β-xylosidase specific activity in terms on an enzyme activity for degrading p-nitrophenyl-β-D-xylopyranoside per 1 mg of protein in said cellulase composition in an amount of 0.006 U/mg protein or less;
- (b) cellobiohydrolase specific activity in terms of an enzyme activity for degrading p-nitrophenyl-β-D-lactopyranoside per 1 mg of protein in said cellulase composition is at least 0.1 U/mg protein;
- (c) β-glucosidase specific activity in terms of an enzyme activity for degrading p-nitrophenyl-β-D-glucopyranoside per 1 mg of protein in said cellulase composition is at least 0.25 U/mg protein;
- and
- (d) the protein concentration of said cellulase composition is 3 g/L or more.
Dependencies are inferred from the claim text.