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US 11,453,895 B1Granted
Engineered hosts with exogenous ligninase and uses thereof
AssigneeNational Technology & Engineering Solutions of Sandia, LLC
InventorsSeema Singh, Arul Mozhy Varman
Life of this patent
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Abstract
The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).
Claims20
An engineered Bacillus host comprising one or more exogenous nucleic acid sequences, wherein at least one exogenous nucleic acid sequence encodes a fungal laccase and a fungal peroxidase;
- wherein the laccase comprises an amino acid sequence having at least 90% sequence identity to one of SEQ ID NOs:
- 1-4, 10, and 11;
- wherein the peroxidase comprises an amino acid sequence having at least 80% sequence identity to one of SEQ ID NOs:
- 20-25;
- and
- wherein the at least one exogenous nucleic acid sequence encodes the peroxidase in a sequence that is codon-optimized for the host.
3 more elements
A method of treating lignin or a derivative thereof, the method comprising:
- providing an engineered microbial host comprising one or more exogenous nucleic acid sequences, wherein at least one exogenous nucleic acid sequence encodes a laccase and a peroxidase;
- and
- introducing the engineered microbial host to a source comprising lignin or a derivative thereof at a pH of from about 4 to 5;
- wherein the laccase comprises an amino acid sequence having at least 90% sequence identity to one of SEQ ID NOs:
- 1-4, 10, and 11;
- wherein the peroxidase comprises an amino acid sequence having at least 80% sequence identity to one of SEQ ID NOs:
- 20-25;
- wherein the host is a member of the genus Bacillus;
- and
- wherein the at least one exogenous nucleic acid sequence encodes the peroxidase in a sequence that is codon-optimized for the host.
7 more elements
A method of treating lignin, the method comprising:
- providing an engineered microbial host comprising one or more exogenous nucleic acid sequences, wherein at least one exogenous nucleic acid sequence encodes a laccase and/or a peroxidase;
- and
- introducing the engineered microbial host to a source comprising lignin at a pH of from about 4 to about 6;
- wherein the source comprises a media comprising a citric acid buffer;
- wherein the host is a member of the genus Bacillus;
- wherein the at least one exogenous nucleic acid sequence encodes at least the peroxidase, the peroxidase comprising an amino acid sequence having at least 99% sequence identity to one of SEQ ID NOs:
- 20-25;
- and
- wherein the at least one exogenous nucleic acid sequence encodes the peroxidase in a sequence that is codon-optimized for the host.
6 more elements
Dependencies are inferred from the claim text.