Methods for delivery of biphasic electrical pulses for non-thermal ablation

A method comprising:

• activating a generator;
• wherein the generator is configured to deliver at least one burst of electrical pulses to a target area at a frequency of at least 40 kHz;
• wherein the at least one burst comprises a first burst of at least five electrical pulses each with a pulse length of 10 μs or less, a second burst of at least five electrical pulses each with a pulse length of 10 μs or less, and there is a time delay of up to 1 second between the first burst and the second burst;
• wherein at least one of the electrical pulses is configured to switch polarity from a positive potential to a negative potential;
• and
• wherein at least one of the electrical pulses is sufficient to non-thermally ablate at least a portion of cells of the target area.

A method comprising:

• activating a generator;
• wherein the generator is configured to deliver at least one burst of electrical pulses to a target area with a frequency of at least 40 kHz;
• wherein the at least one burst comprises a first burst of at least five electrical pulses each with a pulse length of 10 μs or less, and a second burst of electrical pulses of at least five electrical pulses each with a pulse length of 10 μs or less, and there is a time delay of up to 1 second between the first burst and the second burst;
• wherein polarity of a plurality of the electrical pulses is configured to alternate between a positive potential and a negative potential or configured to alternate between a negative potential and a positive potential between the electrical pulses;
• and
• wherein the plurality of electrical pulses are sufficient to non-thermally ablate cells of the target area.