Device and a method for immobilization of proteins, enzymes or cells

A device for preparation of immobilized enzymes on a carrier, comprising:

• i) a conveyor belt that transports a carrier for immobilizing enzymes;
• ii) two or more spraying-kneading components that are arranged in sequence along a transport path of the conveyor belt;
• each of the spraying-kneading components including the following components in sequence:
• (1) a spraying component that sprays a pre-prepared liquid for the immobilization of the enzymes on a surface of the carrier;
• (2) a kneading component that distributes the pre-prepared liquid evenly on the surface of the carrier;
• iii) a squeezing component that removes excess liquid remaining on the surface of the carrier after the carrier passes through the spraying-kneading components,
• wherein each of the kneading components comprises a plurality of kneading rollers arranged above the conveyor belt,
• wherein one or more of the plurality of kneading rollers comprises a smooth surface and one or more of the plurality of kneading rollers comprises uniformly distributed grooves in a surface thereof, and the kneading rollers having smooth surfaces are arranged upstream of the kneading rollers having grooves,
• wherein the kneading rollers are arranged along the transport path of the conveyor belt continuously and in sequence,
• wherein the kneading rollers roll over the surface of the carrier when the carrier passes through a gap between the kneading roller and the conveyor belt;
• wherein the grooves are strip-shaped, a lengthwise direction of the grooves is parallel to an axial direction of the kneading rollers, and the grooves are distributed evenly in a circumferential direction of the kneading rollers, or
• the grooves are ring-shaped, a circumferential direction of the grooves is perpendicular to the axial direction of the kneading rollers, and the grooves are distributed evenly in the axial direction of the kneading rollers, and
• wherein for two adjacent kneading rollers that have the ring-shaped grooves, the ring-shaped grooves of one of the adjacent kneading rollers are offset by a width of one groove with respect to the ring-shaped grooves of the other kneading roller respectively.