Variants and compositions comprising variants with high stability in presence of a chelating agent
AssigneeNovozymes A/S
InventorsAllan Svendsen, Annette Helle Johansen, Mads Bjoernvad, Frank W. Rasmussen, Michael Skjoet, Signe Eskildsen Larsen, Jens Oebro, Svend Kaasgaard, Lars Beier
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Abstract
The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.
Claims23
A variant of a parent alpha-amylase comprising alterations corresponding to positions 206 and 243 of SEQ ID NO:
- 10, wherein the variant has alpha-amylase activity and at least 95% sequence identity to SEQ ID NO:
- 10.
A composition comprising a variant of a parent alpha-amylase, wherein the variant comprises alterations corresponding to positions 206 and 243 of SEQ ID NO:
- 10 and further comprising at least one chelating agent, wherein said chelating agent at a concentration below 10 mM reduces the concentration of free calcium ions from 2.0 mM to 0.10 mM when measured at 21° C. and pH 8.0, and wherein the variant has alpha-amylase activity and at least 95% sequence identity to SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.