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US 11,453,858 B2Granted
Human plasma-like medium
AssigneeWhitehead Institute for Biomedical Research
InventorsDavid M. Sabatini, Jason Cantor
Life of this patent
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Abstract
In some aspects, described herein are cell culture media that are useful for in vitro culture of mammalian cells. The culture media contain a variety of small organic compounds that are found in normal adult human blood. Also described are methods of using the culture media for a variety of purposes. Also described are methods of treating cancer.
Claims26
A basal culture medium, comprising:
- at least 9 proteinogenic amino acids;
- one or more vitamins;
- one or more inorganic ions;
- glucose;
- and
- at least 10 small organic compounds selected from 4-hydroxyproline, acetylglycine, alpha-aminobutyrate, betaine, carnitine, citrulline, ornithine, taurine, 2-hydroxybutyrate, 3-hydroxybutyrate, acetate, citrate, formate, lactate, malonate, pyruvate, succinate, acetone, creatine, creatinine, glutathione, glycerol, urea, galactose, fructose, hypoxanthine, and uric acid.
3 more elements
Dependencies are inferred from the claim text.