Recombinant bifunctional protein targeting CD47 and HER2
AssigneeImmuneonco Biopharmaceuticals (Shanghai) Inc.
InventorsWenzhi Tian, Song Li
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Abstract
The present disclosure provides a recombinant fusion protein containing an extracellular Ig-like domain of a signal-regulator protein (SIRP), linked via a linker, to a paratope of an Ig-like anti-HER2 antibody at the N-terminus of a heavy chain or a light chain constituting the paratope. The present disclosure also provides a polynucleotide encoding the recombinant fusion protein, an expression vector containing the polynucleotide, a method for producing the recombinant protein and a method for treating a disease caused by over-expression of CD47 and/or HER2.
Claims10
A recombinant fusion protein, comprising SEQ ID NO:
- 2, a linker comprising a peptide of 10 to 30 amino acid residues, and an anti-HER2 antibody, wherein every paratope of the anti-HER2 antibody is linked to SEQ ID NO:
- 2 at the N-terminus of the light chain constituting the paratope, and wherein the anti-HER2 antibody heavy chains comprise SEQ ID NO:6 and the anti-HER2 antibody light chains comprise SEQ ID NO:8, and the recombinant fusion protein is capable of blocking binding of CD47s on cancer cells to signal-regulatory proteins (SIRPs) on surfaces of macrophages, binding to HER2s on cancer cells to inhibit uncontrolled cancer cell growth, and binding to FcRs on NK cells or macrophages.
Dependencies are inferred from the claim text.