Protein product and preparation method thereof
AssigneeCofco Nutrition and Health Research Institute Co., Ltd.
InventorsZelong Liu, Benjun Sun, Xiaohui Xiong, Yu Wang, Jia Yang
Life of this patent
Estimating the term…
Abstract
A protein product and an extraction method for making the protein product. Raw materials of the product optionally contains macromolecular carbohydrates and/or fat, and the method does not use organic solvents. The protein product contains prolamin and carbohydrates, wherein the prolamin accounts for 70 wt % or above of the protein (dry-basis), the α-prolamin accounts for 75 wt % or above of the prolamin, the β-prolamin accounts for 20 wt % or below of the prolamin, and the γ-prolamin accounts for 6 wt % or below of the prolamin.
Claims15
A method for increasing the content of α-prolamin from a raw material, the raw material selected from the group consisting of corn gluten meal, corn endosperm fermented mash, and distiller's grains and comprising α-prolamins, β-prolamin, γ-prolamin and non-prolamin, the method comprising:
- milling and slurrying the raw material;
- treating the raw material with protease to completely hydrolyze or partially hydrolyze at least a part of the β-prolamin, the γ-prolamin and the non-prolamin in the raw material and adding a reagent composition, where the protease is one or more selected from the group consisting of Aspergillus oryzae carboxyl endoprotease, Bacillus subtilis serine endoprotease, Bacillus subtilis metalloendoprotease, bromelain, and papain, and the reagent composition comprises one or more selected from the group consisting of the following substances:
- tris(2-carboxvethyl)phosphine, a compound having a free thiol group, a compound having a sulfite group, a metal ion and a metal chelator, wherein the treating is at a pH of 3.5-10.5, a temperature of 20° C-65° C., and for 0.2-10 hours;
- performing first filtration by using a difference in particle size to remove the hydrolysate, thereby obtaining a crude product in which the α-prolamins are enriched, where the first filtration is performed by using a filtration pore size of 1 μm -80 pm or a membrane filtration pore size of 10 nm-10 μm;
- and
- washing, dewatering and drying the crude product to obtain a final product,
- wherein the method does not use organic solvents, the final product comprises prolamins and carbohydrates, where the prolamin accounts for 70 wt % or above of the protein (dry-basis), the α-prolamin accounts for 75 wt % or above of the prolamin, the β-prolamin accounts for 20 wt % or below of the prolamin, and the γ-prolamin accounts for 6 wt % or below of the prolamin.
A method for increasing the content of a-prolamin from a raw material, the raw material comprising α-prolamins, β-prolamin, γ-prolamin and non-prolamin the method comprising:
- milling and slurrying the raw material;
- treating the raw material with protease to completely hydrolyze or partially hydrolyze at least a part of the β-prolamin, the γ-prolamin and the non-prolamin in the raw material and adding a reagent composition, where the protease is one or more selected from the group consisting of Aspergillus oryzae carboxyl endoprotease, Bacillus subtilis serine endoprotease, bromelain, and papain, and the reagent composition comprises one or more selected from the group consisting of the following substances:
- tris(2-carboxvethyl)phosphine, a compound having a free thiol group, a compound having a sulfite group, a metal ion and a metal chelator, wherein the treating is at a pH of 3.5-10.5, a temperature of 20° C.-65° C., and for 0.2-10 hours;
- performing first filtration by using a difference in particle size to remove the hydrolysate, thereby obtaining a crude product in which the α-prolamins are enriched where the first filtration is performed by using a filtration pore size of 1 μm-80 μm or a membrane filtration pore size of 10 nm-10 μm;
- and
- (3) washing, dewatering and drying the crude product to obtain a final product, wherein the method does not use organic solvents, the final product comprises prolamins and carbohydrates, where the prolamin accounts for 70 wt % or above of the protein (dry-basis), the α-prolamin accounts for 75 wt % or above of the prolamin, the β-prolamin accounts for 20 wt % or below of the prolamin, and the γ-prolamin accounts for 6 wt % or below of the prolamin.
Dependencies are inferred from the claim text.