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US 11,453,692 B2Granted
Non-caloric sweeteners and methods for synthesizing
AssigneeConagen Inc.
InventorsGuohong Mao, Xiaodan Yu
Life of this patent
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Abstract
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
Claims18
A method for synthesizing rebaudioside E from rebaudioside KA, the method comprising:
- (i) preparing a reaction mixture comprising (a) at least one of rebaudioside KA and stevioside, (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and (c) a UDP-glycosyltransferase fusion enzyme comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 11;
- (ii) incubating the reaction mixture for a sufficient time to produce rebaudioside E, wherein a glucose is covalently coupled to the C2′-13-O-glucose of rebaudioside KA to produce rebaudioside E.
A method for synthesizing rebaudioside E from rubusoside, the method comprising:
- (i) preparing a reaction mixture comprising (a) rubusoside, (b) substrates selected from the group consisting of sucrose, uridine diphosphate (UDP) and uridine diphosphate-glucose (UDP-glucose), and (c) a UDP-glycosyltransferase fusion enzyme comprising an amino acid sequence having at least 90% identity to SEQ ID NO:
- 11;
- (ii) incubating the reaction mixture for a sufficient time to produce rebaudioside E, wherein a glucose is covalently coupled to rubusoside to produce rebaudioside KA or stevioside, and a glucose is covalently coupled to rebausiode KA or stevioside to produce rebaudioside E.
Dependencies are inferred from the claim text.