Non-invasive prenatal test on single fetal cells isolated from blood of pregnant women
AssigneeLian Liu
InventorsLian Liu, Shuang Hou, Chun-Hao Luo
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Abstract
Methods and systems are provided for isolating fetal cells from a maternal blood supply in order to perform non-invasive prenatal testing. In one example, a system for non-invasive prenatal testing includes a substrate coated with a cell-capturing surface, the cell-capturing surface including an array of pillar-like structures, each pillar-like structure including a plurality of intersecting arms.
Claims9
A method for isolating fetal cells from a maternal blood sample, comprising:
- supplying, by a polymer-coated laser microdissectible (PCLMD) chip, the maternal blood sample to an inlet of a microfluidic channel of a laser micro-dissectible (LMD) chip;
- and flowing, by the PCLMD chip, the maternal blood sample through the microfluidic channel and over a polymer membrane coating one or more surfaces of the microfluidic channel, the polymer membrane comprising an array of structures, wherein the array of structures comprises an array of double-cross pillar structures, each double-cross pillar structure comprising two crosses intersecting at a center region of the double-cross pillar, each cross comprised of two arms of equal length intersecting at the center region, and wherein the double-cross pillar structures bind with the fetal cells to isolate fetal cells from a maternal blood sample.
A method for isolating fetal cells from a maternal blood sample using a system for non-invasive prenatal testing, comprising a substrate coated with a cell-capturing surface, the cell-capturing surface including an array of double-cross pillar structures, each double-cross pillar structure comprising two crosses intersecting at a center region of the double-cross pillar, each cross comprised of two arms of equal length intersecting at the center region, the method comprising the steps:
- supplying the maternal blood sample to an inlet of a microfluidic channel of a laser micro-dissectible (LIVID) chip;
- and flowing the maternal blood sample through the microfluidic channel and over a polymer membrane coating one or more surfaces of the microfluidic channel.
Dependencies are inferred from the claim text.