Chip for separating and capturing cell and application of chip in tumor cell sorting thereof

An integrated chip for separating and capturing a cell with one step, comprising a cell enrichment region, a cell separation region and a cell capture region, wherein one end of the cell enrichment region is provided with one or more inlets, and the other end of the cell enrichment region is provided with a waste liquid outlet and an enriched liquid outlet;

• the cell enrichment region is composed of one, two or more sets of symmetrical Deterministic Lateral Displacement micropost array structures;
• the cell greater than a critical sorting diameter of the symmetrical DLD micropost array structure is enriched to a middle of the symmetrical DLD micropost array structure when flowing through the cell enrichment region, gathers and then flows into the cell separation region;
• and a waste liquid flows out from the waste liquid outlet;
• one end of the cell separation region is provided with a buffer solution inlet and an enriched liquid inlet connecting with the enriched liquid outlet of the cell enrichment region, and the other end of the cell separation region is provided with an outlet;
• one end of the cell capture region is provided with an inlet connecting with the outlet of the cell separation region, and the other end of the cell capture region is provided with a separated liquid outlet;
• and a to-be-separated cell sap flows from the inlet of the cell enrichment region to enter the cell enrichment region;
• the cell enrichment region configured to improve a concentration of a target cell in the cell sap;
• an enriched liquid flowing out from the cell enrichment region and passing through the enriched liquid inlet, and a buffer solution passing through the buffer solution inlet jointly flow into the cell separation region;
• the cell separation region is capable of separating the inflowed cell as per a size;
• the cell separated as per the size enters the cell capture region;
• and the cell capture region is capable of capturing the target cell.