De novo synthesized gene libraries
AssigneeTwist Bioscience Corporation
InventorsWilliam Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
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Abstract
De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.
Claims27
A method for synthesizing oligonucleotides, comprising:
- synthesizing a plurality of oligonucleotides at least 50 nucleotides in length, wherein the synthesizing comprises coupling with nucleosides having a blocking group in an extension reaction, wherein the coupling has a coupling efficiency on average of at least 99.6%, and wherein the plurality of polynucleotides comprise a plurality of different nucleotide bases.
Dependencies are inferred from the claim text.