Tissue stain and use thereof

A liquid tissue staining composition consisting of:

• a thermally sterilizable mixture consisting of
• deagglomerated carbon black-derived carbon particles at a concentration of from about 0.1% to about 0.5% (w/v) having a mean particle diameter from about 0.05 μm to about 0.2 μm;
• a viscosity-increasing agent selected from the group consisting of glycerol, propylene glycol, isopropylene glycol, polyethylene glycol, cellulose, and any combination thereof present at a concentration of from about 10% to about 20% (w/v);
• a surfactant comprising polyoxyethylene sorbitan esterified with fatty acid present at a concentration of about 0.5% to about 2.0% (w/v);
• an antifoaming agent selected from the group consisting of dimethicone, simethicone, and any combination thereof present at a concentration of from about 0.01% to about 0.5% (w/v);
• a preservative selected from the group consisting of benzyl alcohol, methyl paraben, ethyl paraben, benzalkonium chloride, and any combination thereof;
• and,
• water,
• wherein, when the liquid tissue staining composition is injected into a submucosal layer, the resulting mark can be visualized after one month.