Antibacterial composition effective in treating gram negative bacterial infections and method for preparing the same

A method of preparing an antibacterial protein that includes at least one selected from the group of a protein having the amino acid sequence as set forth in SEQ ID NO:

• 1, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 2, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 3, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 4, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 5, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 6, a protein having the amino acid sequence as set forth in SEQ ID NO:
• 7, and a protein having the amino acid sequence as set forth in SEQ ID NO:
• 8:
• culturing Escherichia coli cells including a plasmid that comprises a sequence as set forth in SEQ ID NO:
• 17, SEQ ID NO:
• 18, SEQ ID NO:
• 19, SEQ ID NO:
• 20, SEQ ID NO:
• 21, SEQ ID NO:
• 22, SEQ ID NO:
• 23, or SEQ ID NO:
• 24;
• inducing the expression of the antibacterial protein;
• recovering an inclusion body;
• solubilizing the inclusion body;
• purifying the antibacterial protein;
• and
• refolding the antibacterial protein.