Method of preparing bioactive substance-encapsulated ethosome, ethosome composition, and cosmetic composition including ethosome composition

A method of preparing a bioactive substance-encapsulated ethosome, the method comprising:

• preparing an aqueous solution of a bioactive substance comprising a peptides-hydrogel complex formed by binding an amine group of the peptides and a carboxyl group of the hydrogel, wherein the hydrogel is a carbomer;
• preparing a lipid-dissolved solution by dissolving lipids in ethanol, wherein the lipids comprise phospholipids;
• preparing a hydrated liquid crystalline phase by hydrating the lipid-dissolved solution by mixing and agitating the aqueous solution of the bioactive substance together with the lipid-dissolved solution;
• and
• preparing a bioactive substance-encapsulated ethosome solution by adding purified water to the hydrated liquid crystalline phase to produce a mixture and agitating the mixture, wherein in the step of preparing the ethosome solution, the peptides-hydrogel complex is encapsulated inside the ethosome to generate the bioactive substance-encapsulated ethosome,
• wherein a content of the phospholipids in the ethosome solution is 0.1 wt % to 10 wt %, and wherein a content of the ethanol in the ethosome solution is 2 wt % to 8 wt %, and wherein a content of the hydrogel in the ethosome solution is 0,005 wt %.