System and method for detecting alzheimer's disease

A method for detecting whether a subject having a target suffers from an Alzheimer's disease, comprising steps of:

• imaging the target by a second harmonic generation (SHG) and a third harmonic generation (THG) via a multi-harmonic generation microscope to obtain an SHG image and a THG image;
• adding a first color for the SHG image and a second color for the THG image via a processor to obtain a color-added SHG image and a color-added THG image;
• combining the color-added SHG image and the color-added THG image via the processor to obtain a combined image;
• and
• determining whether the subject suffers from the Alzheimer's disease according to the combined image of the target.

A method for detecting a characteristic of a neural structure, comprising:

• imaging a target by a second harmonic generation (SHG) and a third harmonic generation (THG) via a multi-harmonic generation microscope to obtain an SHG image and a THG image respectively;
• processing the SHG image and the THG image with two different image processing treatments to obtain a processed SHG image and a processed THG image respectively, wherein a combination of the two different image processing treatments highlights the characteristic;
• and
• combining the processed SHG image and the processed THG image to obtain a combined image to determine the characteristic.