Tobacco plants having altered amounts of one or more alkaloids in leaf and methods of using such plants
AssigneeAltria Client Services LLC
InventorsJesse Frederick, Yanxin Shen, Dongmei Xu, Ujwala Warek, James Arthur Strickland
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Abstract
This disclosure provides a number of sequences involved in the transport of alkaloids from the root to the leaf in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences, and tobacco products made from such plants.
Claims13
A tobacco plant comprising an induced mutation in an endogenous gene, wherein a naturally occurring sequence of the endogenous gene has the sequence shown in SEQ ID NO:
- 35, wherein the mutation results in reduced expression of the endogenous gene relative to a corresponding tobacco plant lacking the mutation, and wherein the tobacco plant exhibits reduced transport of nicotine from root tissue to leaf tissue relative to the corresponding tobacco plant lacking the mutation.
A method of making a tobacco plant, comprising the steps of:
- (a) inducing mutagenesis in tobacco cells to produce mutagenized cells;
- (b) obtaining one or more tobacco plants from the mutagenized cells;
- (c) identifying at least one tobacco plant obtained in step (b) that comprises a mutation in an endogenous gene, wherein a naturally occurring sequence of the endogenous gene has the sequence shown in SEQ ID NO:
- 35, wherein the mutation results in reduced expression of the endogenous gene relative to a corresponding tobacco plant lacking the mutation, and wherein the tobacco plant exhibits reduced transport of nicotine from root tissue to leaf tissue relative to leaf from the corresponding tobacco plant lacking the mutation.
A method for producing a tobacco plant, said method comprising the steps of:
- (a) crossing at least one plant of a first tobacco line with at least one plant of a second tobacco line, wherein the at least one plant of the first tobacco line comprises an induced mutation in an endogenous gene, wherein a naturally occurring sequence of the endogenous gene has the sequence shown in SEQ ID NO:
- 35, wherein the induced mutation results in reduced expression of the endogenous gene relative to a corresponding plant lacking the mutation, and wherein the plant of the first tobacco line exhibits reduced transport of nicotine from root tissue to leaf tissue relative to the corresponding tobacco plant lacking the mutation;
- (b) selecting for progeny tobacco plants that comprise the mutation and exhibit reduced expression of the endogenous gene relative to a corresponding tobacco plant lacking the induced mutation;
- and
- (c) selecting for progeny tobacco plants from the progeny tobacco plants selected in step (b) that comprise leaf exhibiting reduced transport of nicotine from root tissue to leaf tissue relative to the corresponding tobacco plant lacking the induced mutation.
A tobacco product comprising cured leaf from a tobacco plant having a mutation in an endogenous gene, wherein said endogenous gene has the sequence shown in SEQ ID NO:
- 35, wherein the mutation results in reduced expression of the endogenous gene relative to a corresponding tobacco plant lacking the mutation, and wherein the the tobacco plant exhibits reduced transport of nicotine from root tissue to leaf tissue relative to the corresponding tobacco plant lacking the mutation.
A method of producing a tobacco product, the method comprising:
- (a) providing cured leaf from a tobacco plant having a mutation in an endogenous gene, wherein said endogenous gene has having the sequence shown in SEQ ID NO:
- 35, wherein the mutation results in reduced expression of the endogenous gene relative to a corresponding tobacco plant lacking the mutation, and wherein the the tobacco plant exhibits reduced transport of nicotine from root tissue to leaf tissue relative to the corresponding tobacco plant lacking the mutation;
- and
- (b) manufacturing a tobacco product using the cured leaf.
Dependencies are inferred from the claim text.