Compositions useful in the diagnostic of latently infected Mycobacterium tuberculosis
AssigneesCnrs, Conicet, Institut Jean Paoli & Irene Calmetes, Université d' Aix-Marseille, Inserm
InventorsJuan Iovanna, Virginia Pasquinelli, Maria Madgalena Gherardi, Hector Eduardo Chuluyan, Ana Inès Rovetta, Delfina Pena, Véronica Edith Garcia
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Abstract
The present invention concerns a composition comprising at least three peptides derived from Mycobacterium tuberculosis antigen Rv2626c, its use in the diagnostic of latently infected Mycobacterium tuberculosis (LTBI) subjects, corresponding methods of use and kits.
Claims2
A method of diagnosing latently infected Mycobacterium tuberculosis (LTBI) in a human subject, said method comprising:
- (i) culturing isolated peripheral blood mononuclear cells (PBMC) or a blood sample from the subject with a composition, wherein the composition comprises peptide 1 consisting of SEQ ID NO:
- 25, peptide 2 consisting of SEQ ID NO:
- 26, and peptide 5 consisting of SEQ ID NO:
- 29;
- and/or peptide 12 consisting of SEQ ID NO:
- 3, peptide 23 consisting of SEQ ID NO:
- 17, and peptide 24 consisting of SEQ ID NO:
- 5;
- and
- (ii) measuring the level of expression of IFN-gamma of said cultured PBMC or said cultured blood sample;
- and
- (iii) diagnosing the LTBI in said human subject when the level of the IFN-gamma in said human subject is higher compared to the level of IFN-gamma in healthy human subjects and human patients with active tuberculosis.
A method of discriminating a human subject with LTBI from a healthy human subject and from a human patient with active tuberculosis comprising:
- (i) culturing isolated peripheral blood mononuclear cells (PBMC) or a blood sample from the subject with a composition, wherein the composition comprises peptide 1 consisting of SEQ ID NO:
- 25, peptide 2 consisting of SEQ ID NO:
- 26, and peptide 5 consisting of SEQ ID NO:
- 29;
- and/or peptide 12 consisting of SEQ ID NO:
- 3, peptide 23 consisting of SEQ ID NO:
- 17, and peptide 24 consisting of SEQ ID NO:
- 5, wherein the composition further comprises CFP-10 antigen and/or ESAT antigen of Mycobacterium tuberculosis;
- (ii) measuring the level of expression of IFN-gamma of said cultured PBMC or said cultured blood sample;
- and
- wherein a higher level of the IFN-gamma in said human subject compared to the level of IFN-gamma in the healthy human subject and in the human patient with active tuberculosis discriminates the human subject with LTBI from the healthy human subject and the human patient with active tuberculosis.
Dependencies are inferred from the claim text.