Method for cancer prognosis
AssigneeUniversity of Technology, Sydney
InventorsMary Bebawy, Sabna Rajeev Krishnan
Life of this patent
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Abstract
The present invention relates to methods of prognosing and monitoring cancer using circulating cells and/or extracellular vesicles as indicators of the progression of plasma cell neoplasms in patients.
Claims20
A method for the prognosis of a patient with a plasma cell neoplasm or suspected of having a plasma cell neoplasm, comprising isolating a sample comprising extracellular vesicles, or cells and extracellular vesicles, from said patient and determining the level of extracellular vesicles, or cells and extracellular vesicles, that are CD138− and P-glycoprotein+ (CD138−/P-gp+), wherein the determining step comprises using an immunoaffinity method, wherein antibodies are directed towards a cell surface and/or microparticle-associated proteins;
- and wherein the immunoaffinity methods comprise using affinity pull-downs, Western blot analysis, dot blot analysis, radio-immune assays, flow cytometry, fluorescence activated cell sorting (FACS) analysis, magnetic beads, ELISA, immunofluorescence and/or affinity chromatography.
A method for monitoring the progression of a patient with a plasma cell neoplasm or suspected of having a plasma cell neoplasm, comprising isolating a sample comprising extracellular vesicles, or cells and extracellular vesicles, from said patient at at least two time points and determining the change in the level of extracellular vesicles, or cells and extracellular vesicles, that are CD138−/P-gp+, wherein the determining step comprises using an immunoaffinity method, wherein antibodies are directed towards a cell surface and/or microparticle-associated proteins;
- and wherein the immunoaffinity methods comprise using affinity pull-downs, Western blot analysis, dot blot analysis, radio-immune assays, flow cytometry, fluorescence activated cell sorting (FACS) analysis, magnetic beads, ELISA, immunofluorescence and/or affinity chromatography.
Dependencies are inferred from the claim text.