Methods of assaying proteins
AssigneeNautilus Biotechnology, Inc.
InventorParag Mallick
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Abstract
Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.
Claims24
A flowcell, comprising:
- at least a first surface on a substrate disposed within the flowcell;
- a plurality of polypeptide molecules from a biological sample immobilized on the at least first surface, wherein the at least first surface comprises at least 1,000,000 individual polypeptide molecules immobilized on the at least first surface, each of the at least 1,000,000 individual polypeptide molecules being positioned to be individually spatially resolvable from each other individual polypeptide on the first surface;
- wherein the plurality of polypeptide molecules immobilized on the first surface comprises a plurality of different polypeptides present on the first surface, and wherein the number of first polypeptides immobilized on the surface corresponds to the level of the first polypeptides in the biological sample;
- and
- wherein the polypeptide molecules are attached to the first surface of the substrate through a nucleic acid attachment.
Dependencies are inferred from the claim text.