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US 11,448,642 B2Granted
Method for identifying malodour counteractants
AssigneeTakasago International Corporation
InventorsJonathan Warr, Marcel Winnig
Life of this patent
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Abstract
The invention relates to a method for determining whether a test compound can counteract malodour from a sulphur odorant. The method includes contacting the test compound and the sulphur odorant with an olfactory receptor OR4E2, and comparing the binding of the OR4E2 to the sulphur odorant, or the activity of the OR4E2, in the presence and in the absence of the test compound.
Claims9
A method for determining whether a test compound can counteract a perceived malodour from a sulfur odorant, the method comprising the steps of:
- a) contacting cells expressing an OR4E2 polypeptide with the sulfur odorant in the presence and in the absence of the test compound;
- b) measuring an activity of the OR4E2 polypeptide in the presence and in the absence of the test compound under an identical condition;
- c) comparing the activity of the OR4E2 polypeptide in the presence of the test compound to the activity of the OR4E2 polypeptide in the absence of the test compound;
- wherein a decrease in the activity of the OR4E2 polypeptide in the presence of the test compound relative to the activity of the OR4E2 polypeptide in the absence of the test compound identifies the test compound as a compound that can counteract the perceived malodour from the sulfur odorant;
- wherein the sulfur odorant is selected from the group consisting of:
- a sulfanyl alkanol selected from the group consisting of 2-mercapto-3-methyl-1-butanol, 3-mercapto-2-methyl-1-pentanol, 3-mercapto-2-methyl-1-propanol, 3-mercapto-3-methyl-1-butanol, 3-mercapto-1-hexanol, 3-methyl-3(2-methyldisulfanyl)-butan-1-ol and 3-mercapto-3-methyl-1-hexanol;
- and a sulfide selected from the group consisting of methyl-2-propenyl disulfide, 2,4-dithiapentane, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, diallyl sulfide, diallyl disulfide and diallyl trisulfide;
- and
- wherein the activity of the OR4E2 polypeptide is measured using a reporting system capable of producing a readable or measurable signal.
6 more elements
A method for determining whether a test compound can counteract a perceived malodour from a sulfur odorant, the method comprising the steps of:
- a) providing cells expressing an OR4E2 polypeptide;
- b) measuring a magnitude of a response of the OR4E2 polypeptide to control conditions;
- c) contacting said cells with the sulfur odorant in the presence and in the absence of the test compound under an identical condition;
- d) measuring a magnitude of a response of the OR4E2 polypeptide to the sulfur odorant in the absence of the test compound under an identical condition;
- e) calculating a first fold of change (FOC1), wherein FOC1 equals the magnitude of the response measured in step d) divided by the magnitude of the response measured in step b);
- f) measuring a magnitude of a response of the OR4E2 polypeptide to the sulfur odorant in the presence of the test compound under an identical condition;
- g) calculating a second fold of change (FOC2), wherein FOC2 equals the magnitude of the response measured in step f) divided by the magnitude of the response measured in step b);
- h) comparing FOC1 with FOC2;
- and
- i) identifying the test compound as a compound that can counteract the perceived malodour from the sulfur odorant if a ratio FOC2/FOC1 is less than 0.8;
- wherein the sulfur odorant is selected from the group consisting of:
- a sulfanyl alkanol selected from the group consisting of 2-mercapto-3-methyl-1-butanol, 3-mercapto-2-methyl-1-pentanol, 3-mercapto-2-methyl-1-propanol, 3-mercapto-3-methyl-1-butanol, 3-mercapto-1-hexanol, 3-methyl-3(2-methyldisulfanyl)-butan-1-ol and 3-mercapto-3-methyl-1-hexanol;
- and a sulfide selected from the group consisting of methyl-2-propenyl disulfide, 2,4-dithiapentane, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, diallyl sulfide, diallyl disulfide and diallyl trisulfide.
10 more elements
Dependencies are inferred from the claim text.