Biodetector based on interference effect of thin film with ordered porous nanostructures and method for using same to detect biomolecules

A biodetector based on an interference effect of a thin film with ordered porous nanostructures, the biodetector comprising:

• a detection cell, comprising a substrate;
• a sensing unit disposed in the detection cell and on the substrate, wherein the sensing unit is an ordered porous thin film provided with three-dimensional ordered nanopores, the ordered porous thin film is transparent, and diameters of the nanopores are 20 nm-500 nm;
• an optical fiber spectrometer for detecting a reflectometric interference spectrum of the sensing unit;
• and
• a control system for controlling the optical fiber spectrometer and analyzing the reflectometric interference spectrum to obtain an optical thickness of the sensing unit,
• wherein the control system to analyze the reflectometric interference spectrum to obtain an optical thickness change of the sensing unit is configured to obtain the reflectometric interference spectrum of the sensing unit;
• and use a single peak fitting method or a multi-peak fitting method to obtain a time dependent curve of the optical thickness of the sensing unit according to the reflectometric interference spectrum,
• wherein the nanopores are spherical, and any two adjacent nanopores of the nanopores penetrate with each other,
• wherein the sensing unit has a top and a bottom opposite the top, a sample to be detected enters the nanopores from the top of the sensing unit, and the substrate contacts the bottom of the sensing unit,
• wherein the optical fiber spectrometer comprises a broad frequency light source, a Y-type optical fiber bundle, a reflected light probe, an entrance slit, a grating, and a detector;
• and the reflected light probe is disposed under the sensing unit and adjacent to the bottom of the sensing unit, so that the reflectometric interference spectrum can be detected by the reflected light probe from the bottom of the sensing unit.