Compositions and methods for rapid production of versatile nanobody repertoires
AssigneeThe Rockefeller University
InventorsMichael P. Rout, Brian T. Chait
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Abstract
Provided are compositions and methods for producing large repertoires of recombinant nanobodies with high affinities and specificities against any antigen. Included are methods for making and identifying nanobodies produced by camelids, the nanobodies themselves, modifications of the nanobodies, expression vectors encoding the nanobodies, cDNAs encoding the nanobodies, cells comprising the expression vectors and/or cDNA, and methods of making the nanobodies recombinantly. Antigen-specific nanobodies and antigen binding fragments thereof having a Kd for the antigen in a sub-micromolar range are provided.
Claims2
A method of detecting a protein comprising green fluorescent protein (GFP) comprising contacting a sample containing or suspected of containing the GFP with a dimer comprising distinct two distinct heavy chain antibodies, comprising SEQ ID NO:21 (LaG-16) and SEQ ID NO:10 (LaG2), wherein the dimer comprises a glycine-rich linker between the LaG-16 and LaG2, and detecting a complex of the dimer and the protein comprising the GFP.
Dependencies are inferred from the claim text.