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US 11,447,835 B2Granted
Compositions and methods for detecting or quantifying hepatitis C virus
AssigneeGen-Probe Incorporated
InventorsSiobhan Miick, Paul M. Darby, Jo Ann Jackson, Sheila M. J. Aubin
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Abstract
This disclosure provides oligomers, compositions, and kits for detecting and quantifying Hepatitis C virus (HCV), including different genotypes and variants thereof, and related methods and uses. In some embodiments, oligomers target the 5′ untranslated region of HCV and are configured to provide substantially equivalent quantification of different genotypes and variants of HCV.
Claims21
A composition or kit comprising:
- at least first and second amplification oligomers, wherein:
- the first amplification oligomer comprises a target-hybridizing sequence comprising at least 10 contiguous nucleotides of SEQ ID NO:
- 2, including at least a portion of the nucleotide sequence containing positions 5, 7, 12, and 15 of SEQ ID NO:
- 2;
- and
- the second amplification oligomer comprises a target-hybridizing sequence comprising at least 10 contiguous nucleotides of SEQ ID NO:
- 3 including at least a portion of the nucleotide sequence containing positions 5, 7, 12, and 15 of SEQ ID NO:
- 3;
- the target-hybridizing sequences of the first and second amplification oligomers each comprise at least about 14 contiguous nucleotides of Hepatitis C virus sequence;
- and
- the kit or composition comprises at least one of:
- (i) an amplification oligomer that is a promoter-primer;
- or (ii) a probe oligomer, wherein the probe oligomer comprises a non-nucleotide detectable label and/or wherein at least about half of the sugar moieties in the probe oligomer are 2′-O-methyl-ribose.
10 more elements
Dependencies are inferred from the claim text.