Genetic array for simultaneous detection of multiple Salmonella serovars
AssigneeTuskegee University
InventorsWoubit Abebe, Khaled Aldhami, Sayma Afroj, Temesgen Samuel, Gopal Reddy
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Abstract
Disclosed are novel genetic arrays for use in the molecular detection of multiple Salmonella serovars, common food-borne and water-borne pathogens. The arrays may be used to simultaneously detect multiple food safety Salmonella serovars. The multiplex-detection methods have improved sensitivity and specificity for the detection of multiple high-impact food-borne pathogens simultaneously. Real-time PCR assaying techniques using such serovars include microarrays.
Claims4
A multiplex real-time PCR system for use in simultaneously testing for a plurality of Salmonella serovars that are foodborne pathogens or food threat agents, said system comprising:
- a container for receiving test samples, said container containing two or more primer pairs, said two or more primer pairs being adapted to detect and distinguish with specificity DNA from at least two different Salmonella serovars that are foodborne pathogens or food threat agents,
- wherein all said primer pairs have similar melting temperatures such that they can be simultaneously run under the same polymerase chain reaction (PCR) conditions, and wherein said primer pairs comprise at least one primer selected from SEQ ID NO:
- 1 and SEQ ID NO:
- 2,
- wherein the container further contains a different oligonucleotide probe for each primer pair, the oligonucleotide probes each being designed to bind to DNA regions flanked by the regions in genomes of the at least two different Salmonella serovars targeted by the primer pairs, each probe labeled with a different reporter dye having emission capabilities distinguishable from other ones of said probes.
A method for detecting Salmonella serovars in a sample, said method comprising:
- performing polymerase chain reaction (PCR) using an array for testing a sample simultaneously for a plurality of Salmonella serovars that are foodborne pathogens or food threat agents, said array comprising:
- two or more primer pairs placed on a microplate for receiving test samples, said two or more primer pairs being adapted to detect and distinguish with specificity DNA from at least two different Salmonella serovars that are foodborne pathogens or food threat agents,
- wherein all said primer pairs have similar melting temperatures such that they can be simultaneously run under the same polymerase chain reaction (PCR) conditions, and wherein said primer pairs comprise at least one primer selected from SEQ ID NO:
- 1 and SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.