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US 11,447,822 B2Granted
Nucleic acid constructs and methods of use
AssigneePrognosys Biosciences, Inc.
InventorMark S. Chee
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Abstract
The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.
Claims24
A method for identifying a sequence variant in a sample, comprising:
- (a) contacting a sample with a primer configured to amplify a region of interest (ROI) in a genome, wherein the sample comprises a plurality of nucleic acid templates for the primer and each nucleic acid template is uniquely identified by a unique identifier oligonucleotide, under conditions for extension of the primer to form a family of nucleotide sequences from each uniquely identified nucleic acid template;
- and
- (b) identifying a nucleotide sequence as accurately representing a uniquely identified nucleic acid template when the sequence is a consensus sequence of a plurality of members of a family,
- wherein a sequence variant in the sample is identified when the nucleotide sequence that accurately represents the uniquely identified nucleic acid template is different from a reference sequence.
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Dependencies are inferred from the claim text.