Nucleic acid amplification and testing
AssigneeCambridge Enterprise Limited
InventorsHelen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
Life of this patent
Estimating the term…
Abstract
Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.
Claims22
A method of nucleic acid amplification, comprising amplifying a target nucleic acid by a self-sustained transcription-based amplification reaction which is carried out at a temperature less than 50° C., and in the presence of a reconstituted lyophilised formulation, wherein the lyophilised formulation comprises a polysaccharide, a low molecular weight saccharide, and enzyme activities required for carrying out the self-sustained amplification reaction, wherein the enzyme activities are provided by one or more enzymes, and wherein the lyophilised formulation does not include cofactors or substrates required for amplification of the target nucleic acid using the enzyme activities.
A method of nucleic acid amplification, comprising amplifying a target nucleic acid by a self-sustained transcription-based amplification reaction which is carried out at a temperature less than 50° C., and in the presence of a reconstituted lyophilised formulation, wherein the lyophilised formulation comprises a polysaccharide, a low molecular weight saccharide, and enzyme activities required for carrying out the self-sustained amplification reaction, wherein the enzyme activities are provided by one or more enzymes, and wherein the lyophilised formulation does not include a buffering agent.
A method of nucleic acid amplification, comprising amplifying a target nucleic acid by a self-sustained transcription-based amplification reaction which is carried out at a temperature less than 50° C., and in the presence of a polysaccharide, a low molecular weight saccharide, and enzyme activities required for carrying out the self-sustained amplification reaction, wherein the enzyme activities are provided by one or more enzymes, wherein the formulation does not include cofactors or substrates required for amplification of a target nucleic acid using the enzyme activities, and wherein the formulation does not include a buffering agent, or the buffering agent is present at a concentration of less than 1 mM.
A method of nucleic acid amplification, comprising
- reconstituting a lyophilized formulation, the lyophilised formulation comprising a polysaccharide, a low molecular weight saccharide, and enzyme activities required for carrying out a self-sustained amplification reaction, wherein the enzyme activities are provided by one or more enzymes, and wherein the lyophilised formulation does not include a buffering agent;
- and
- amplifying a target nucleic acid by a self-sustained transcription-based amplification reaction which is carried out at a temperature less than 50° C., and in the presence of the reconstituted lyophilised formulation.
Dependencies are inferred from the claim text.