Single-strand binding protein
AssigneeUniversitetet I Tromsø—Norges Arktiske Universitet
InventorsMarcin Pierechod, Nils Peder Willassen, Ulli Rothweiler
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Abstract
The present invention relates to the use of a single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions, to dehybridize a DNA molecule or to prevent hybridisation of a complementary ssDNA, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the DNA molecule or ssDNA is present in or exposed to a solution containing one or more of the following (i) at least 350 mM of sodium ions; (ii) at least 50 mM of potassium ions; (iii) at least 150 mM of magnesium ions; or (iv) at least 200 mM of calcium ions.
Claims11
A isolated single-strand DNA binding protein (SSB) which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions and comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical thereto, or a functional fragment thereof, and wherein the amino acid at position 17 and/or 71 has been substituted with an amino acid that lacks a negative charge on its side chain.
A composition comprising an isolated SSB which exhibits at least 50% of its maximum ssDNA binding capability in the presence of 500 mM of sodium ions and a buffer and optionally a stabilizing agent, wherein the SSB comprises the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least 75% identical to SEQ ID NO:1, or a functional fragment thereof, and wherein the amino acid at position 17 and/or 71 of the SSB has been substituted with an amino acid that lacks a negative charge on its side chain.
Dependencies are inferred from the claim text.