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US 11,447,810 B2Granted
Compositions and methods for the production of compounds
AssigneeGinkgo Bioworks, Inc.
InventorsBrian R. Bowman, Joshua A. V. Blodgett, Gregory L. Verdine, Daniel C. Gray, Jay P. Morgenstern, Lucy Foulston, Keith Robison
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Abstract
The present disclosure provides nucleic acids encoding a Large ATP-binding regulator of the LuxR family (LAL) of transcription factors, vectors and host cells including such nucleic acids, and methods for producing compounds (e.g., polyketides or β-lactam compounds) with such nucleic acids, vectors, and/or host cells.
Claims19
A method of producing a compound, the method comprising:
- (a) providing a genetically modified host cell comprising:
- (i) a nucleic acid encoding a recombinant Large ATP-binding regulator of the LuxR family (LAL) that is heterologous to the host cell;
- and
- (ii) a nucleic acid comprising an LAL binding site that is heterologous to the host cell, wherein the LAL binding site is operably linked to an open reading frame encoding a compound-producing protein, and wherein binding of the recombinant LAL to the LAL binding site promotes expression of the compound-producing protein;
- and
- (b) culturing the host cell under conditions suitable to allow expression of a compound by the compound-producing protein;
- thereby producing a compound.
4 more elements
Dependencies are inferred from the claim text.