Back to results
US 11,447,809 B2Granted
Evolution of tRNA synthetases
AssigneePresident and Fellows of Harvard College
InventorsDavid R. Liu, David Irby Bryson, Jr.
Life of this patent
Estimating the term…
Abstract
The disclosure provides amino acid sequence variants of orthogonal aminoacyl-tRNA synthetases (AARSs) having increased activity and selectivity compared to previous AARSs, and methods of producing the same.
Claims8
A tRNA synthetase protein variant comprising an amino acid sequence that is at least 85% identical to SEQ ID NO:
- 20 or SEQ ID NO:
- 21, and includes at least one mutation at a position selected from V31, T56, H62, and A100.
A chimeric pyrrolysyl-tRNA synthetase (PylRS) protein variant comprising:
- (i) a first portion comprising amino acid residues 1-149 of Methanosarcina barkeri PylRS (SEQ ID NO:
- 20);
- and
- (ii) a second portion comprising amino acid residues 185-454 of Methanosarcina mazei PylRS (SEQ ID NO:
- 21), wherein the first portion or the second portion comprises at least one of the amino acid substitutions set forth in Tables 2-6.
2 more elements
Dependencies are inferred from the claim text.