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US 11,447,807 B2Granted
Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Evan R. Daugharthy, Richard C. Terry
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Abstract
The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.
Claims22
A method for processing biomolecules within a biological sample, comprising:
- (a) forming a three-dimensional (3D) polymer matrix comprising (i) a swelling agent;
- and (ii) said biomolecules within said biological sample, wherein said 3D polymer matrix preserves a relative 3D spatial relationship of said biomolecules within said biological sample;
- and
- (b) applying a stimulus to said swelling agent to yield an expanded 3D polymer matrix comprising said biomolecules, wherein said stimulus is an electromagnetic radiation stimulus, an electrochemical stimulus, or a thermal stimulus.
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Dependencies are inferred from the claim text.