Anaplerotic oil production in microbials
AssigneeHeliae Development, LLC
InventorsEneko Ganuza Taberna, Magdalena Amezquita Ernult, Charles Sellers
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Abstract
Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.
Claims19
A method for producing anaplerotic oil from microalgae, the method comprising:
- adding an Aurantiochytrium microalgae to a culture medium;
- adding propionate and a carbon source to the culture medium resulting in cultured microalgae with enhanced synthesis of saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) odd-chain fatty acids (OFCAs) and increased total amounts of tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) present in the microalgae;
- and
- obtaining an anaplerotic oil from the cultured microalgae, wherein at least five percent of the total fatty acids (TFAs) of the anaplerotic oil are saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFAs and the saturated tridecanoic (C13:0), pentadecanoic (C15:0), and heptadecanoic (C17:0) OCFAs make up at least one percent of the cell dry weight (CDW) of the microalgal mass,
- wherein:
- the propionate is taken up and used by the Aurantiochytrium microalgae as a precursor for production of the OCFAs;
- and
- the carbon source is taken up and used by the Aurantiochytrium microalgae to support growth of the microalgal mass.
Dependencies are inferred from the claim text.