Microorganisms and processes for lactic acid production
AssigneePTT Global Chemical Public Company Limited
InventorsSudhanshu Vijay Dole, Theron Hermann, Sean Joseph Regan, Mark Andrew Sheff, Russell Lizardo Udani, R. Rogers Yocum
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Abstract
Yeast strains and fermentation process for producing D-lactic acid and L-lactic acid are disclosed with higher titer, higher yield, shorter time, lower pH, and higher average specific productivity.
Claims10
A genetically engineered yeast strain of genus Kluyveromyces to produce lactic acid comprising a chromosomally integrated gene that encodes an exogenous lactate dehydrogenase, wherein said yeast strain produces lactic acid in a fermentation production medium with an average specific productivity of at least 1.875 g/L-hr, and wherein said fermentation production medium has a final pH that is lower than 3.86, wherein said gene that encodes an exogenous lactate dehydrogenase is integrated at least at one chromosomal locus selected from a group consisting of a pyruvate decarboxylase gene, a phosphoenolpyruvate carboxykinase gene, a glycerol-3-phosphate phosphatase gene, and a NADH dehydrogenase 1 gene.
Dependencies are inferred from the claim text.